Van Mour was a native of Valenciennes, a Flemish town that at the time of his birth belonged to the Spanish Netherlands, but since 1678 to France. He studied art in the studio of Jacques-Albert Gérin, and his work attracted the attention of an aristocrat and statesman of the time, Marquis Charles de Ferriol. Van Mour accompanied the De Ferriol to Constantinople after the Marquis appointment as the French Ambassador in 1699. It was there that De Ferriol commissioned Van Mour to do one hundred oil paintings of the local people.
After De Ferriol returned to France in 1711, Van Mour worked for a variety of other diplomats in the Ottoman Empire. A series of one hundred engravings were created after Van Mours portraits, which was published by Le Hay in 1714, titled as Recueil de cent estampes représentant différentes nations du Levant. The book had a great influence in Western Europe and was published in at least five languages. The wide-distribution and popular reception of the Recueil de cent estampes led to an increased recognition of Van Mour as an artist.